zarrin77
Member
Great study showing that methylene blue and low level laser therapy (LLLT) can have further benefits when combined.
Effects of transcranial photobiomodulation and methylene blue on biochemical and behavioral profiles in mice stress model
There are some important things to note. The dose of methylene blue was 0.5 mg/kg i.p. This comes out to a human dose of 2.7-3.5 mg if you inject through your peritoneum. Some chemicals have similar bioavailability orally as they do i.p., some don’t (some require 4x as much orally).
Since I’ve read that oral bioavailability of MB is 73% (someone correct me if this is wrong), that brings the oral human dose to likely ~3.7 - 4.8 mg / day, depending on your size.
Also, the methylene blue was injected 2 hours after the LLLT. This is important, as performing LLLT while MB is already in your tissues could lead to potential oxidative damage and the LLLT could by “synergized”. The authors knew this, and that’s why they gave the MB after LLLT.
Here are the specs for the laser therapy:
“A diode GaAlAs laser (Thor Photomedicine, Chesham, UK) at 810-nm wavelength was applied for irradiation sessions. The laser’s operation mode was Pulsed Wave (PW) on 10 Hz, 88% duty cycle, and average irradiance of 4.75 W/ cm2. A fluence of 8 J/cm 2was delivered to the cortical surface”
They gave the mice LLLT 3 times a week.
They stressed the mice using chronic unpredictable stress for 4 weeks, and at the same time they gave the ratrs either LLLT (tPBM - trascranial photobiomodulation), MB, or both.
Here are some of the results. First image represents “anxiety”, or rather, lack thereof.
full study attached.
Effects of transcranial photobiomodulation and methylene blue on biochemical and behavioral profiles in mice stress model
There are some important things to note. The dose of methylene blue was 0.5 mg/kg i.p. This comes out to a human dose of 2.7-3.5 mg if you inject through your peritoneum. Some chemicals have similar bioavailability orally as they do i.p., some don’t (some require 4x as much orally).
Since I’ve read that oral bioavailability of MB is 73% (someone correct me if this is wrong), that brings the oral human dose to likely ~3.7 - 4.8 mg / day, depending on your size.
Also, the methylene blue was injected 2 hours after the LLLT. This is important, as performing LLLT while MB is already in your tissues could lead to potential oxidative damage and the LLLT could by “synergized”. The authors knew this, and that’s why they gave the MB after LLLT.
Here are the specs for the laser therapy:
“A diode GaAlAs laser (Thor Photomedicine, Chesham, UK) at 810-nm wavelength was applied for irradiation sessions. The laser’s operation mode was Pulsed Wave (PW) on 10 Hz, 88% duty cycle, and average irradiance of 4.75 W/ cm2. A fluence of 8 J/cm 2was delivered to the cortical surface”
They gave the mice LLLT 3 times a week.
They stressed the mice using chronic unpredictable stress for 4 weeks, and at the same time they gave the ratrs either LLLT (tPBM - trascranial photobiomodulation), MB, or both.
Here are some of the results. First image represents “anxiety”, or rather, lack thereof.
full study attached.