Induction of alkaline phosphatase (AP) activity in Ishikawa cells indicates an estrogenic response, whereas inhibition represents an anti-estrogenic effect. However, unlike other estrogenic plant extracts such as red clover and hops, which showed AP induction, neither the methanol extract of chaste berry or linoleic acid stimulated or inhibited AP activity.
Isolation of linoleic acid as an estrogenic compound from the fruits of Vitex agnus-castus L. (chaste-berry)
Alkaline phosphatase activity in human endometrial cancer cells of the estrogen-responsive Ishikawa line was markedly stimulated (3-20-fold in 4 days) by estrogens, DHT, and DHEA but not by testosterone, medroxyprogesterone acetate, glucocorticoids, several peptide hormones, prostaglandins, or growth factors.
Effects of steroid hormones and antisteroids on alkaline phosphatase activity in human endometrial cancer cells (Ishikawa line)