Ok.
By the way how do you convert in vitro doses to in vivo?
From the little I've read it seems very tricky if accurate at all. Do you just use total blood volume ?
I saw an interesting study on how to convert doses in animal studies to humans but that's about it Dose translation from animal to human studies revisited. - PubMed - NCBI .
I could be wrong but it makes sense that depending on where dmso gets absorbed the cells will be exposed to different concentrations , so that if applied on testicles dmso will have a higher concentration in those cells than in the stomach. Maybe the dilution happens quick enough to make the issue irrelevant (except for the skin which does seem to suffer).
Google for "total volume of distribution". The calculation from in vitro to in vivo is involved and likely different for different chemicals. As a quick and dirty rule you can take your weight and use that as a (approximate surrogate) of volume. So, if you weigh 65kg, you can take 65L as the volume of distribution. Like I said, this is a very rough approximation and the actual volume of distribution is different for various chemicals. For instance, something lipophilic like pregnenolone or androsterone or vitamin E will have very high uptake in tissues and thus higher total volume of distribution. But the 65L would be a decent upper limit for any substance you ingest. Clearly, using that upper limit would overstimate the required in vivo dose to achieve the in vitro concentration, so YMMV. I think you can guestimate the volume of distribution of each substance based on its lipophilicity compared to a reference compound like cholesterol, for which I think the volume of distribution is known. So, if something is say only 80% as soluble in oil as cholesterol you can take the volume of distribution for that chemical to be 0.8* max_volume_of_distribution (which in this case is 65L). Obviously other factors such as metabolic speed, liver health, etc come into play as that would determine how much actual chemical reaches the blood. Some people glucuronidate extremely well and very little reaches the blood. Others are slow xenometabolizers and a lot more will reach and stay in the blood.
Anyways, I think you get the idea.