“iron overload decreased renal expression of klotho at both the mRNA and the protein level, and iron chelation suppressed the angiotensin II-induced downregulatoon of this gene, suggesting that alteration in iron metabolismma yhave a role in the angiotensin II-induced downregulationof renalklothoexpression. Furthermore, a free radical scavenger also suppressed the angiotensin II-induced downregula-tion ofklotho, supporting the involvement of an increased production of reactive oxygen species in this process.”